FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
ash2, Set1
FlyBase ID
FBig0000091101
Interaction Network
Interactions Browser links
ash2 network
Set1 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0216343-1.coIP.MS
Reference
Mohan et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
ash2 

Ash2

bait

FLAG-HA tag

Set1 

dSet1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0217167-1.CS.WB
Reference
Hallson et al., 2012
Description
physical association
Assay
Collection
Source/Stage
organism / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Set1 

dSet1

neutral component

FLAG tag

Set1 

dSet1

neutral component

FLAG tag

Set1 

dSet1

neutral component

FLAG tag

ash2 

Ash2

neutral component

HA tag

ash2 

Ash2

neutral component

HA tag

ash2 

Ash2

neutral component

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Adult nuclear extracts were fractionated on a glycerol gradient.

(Hallson et al., 2012)

Set1 and ash2 proteins cosediment as part of a 667 kDa complex, which correlates closely to the additive mass of Drosophila COMPASS complex members (610 kDa).

(Hallson et al., 2012)

Proteins derived from a transgenic line carrying a HA-tagged UAS-ash2 construct, P{UAS-ash2.HA}, and a FLAG-tagged UAS-Set1 construct, P{UAS-Set1.H}.

(Hallson et al., 2012)
FBrf0217167-2.coIP.WB
Reference
Hallson et al., 2012
Description
physical association
Assay
Collection
Source/Stage
organism / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ash2 

Ash2

bait

HA tag

Set1 

dSet1

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Adult nuclear extracts were fractionated on a glycerol gradient.

(Hallson et al., 2012)

Interact as part of the COMPASS complex.

(Hallson et al., 2012)

Proteins derived from a transgenic line carrying a HA-tagged UAS-ash2 construct, P{UAS-ash2.HA}, and a FLAG-tagged UAS-Set1 construct, P{UAS-Set1.H}.

(Hallson et al., 2012)
FBrf0217167-4a.PD.WB
Reference
Hallson et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ash2 

Ash2

bait

GST tag

Set1 

dSet1

prey

HA tag

Set1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Set1 
catalytic C-terminal region
unspecified role
aa 1329-1641
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected HEK293 cell extract.

(Hallson et al., 2012)

ash2 and Set1 interact as part of the COMPASS complex.

(Hallson et al., 2012)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)