Interaction in vitro; proteins derived from S2 nuclear extract.

These proteins coimmunoprecipitate with Ada2a and cofractionate with each other in size exclusion chromatography as part of 1.3-1.8 MDa ATAC histone acetyltransferase complex.

TAP-tagged Ada2a was affinity purified and co-purifying proteins were analyzed by gel filtration.

Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

Tandem affinity purification of bait and identification of prey by MudPIT.