FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Dis3, Rrp42
FlyBase ID
FBig0000094263
Interaction Network
Interactions Browser links
Dis3 network
Rrp42 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0210480-1.coIP.WB
Reference
Graham et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Dis3 

dDis3

bait

FLAG tag

Dis3 
Dis3 
Dis3 
Dis3 
Dis3 
Dis3 
Rrp42 

dRrp42

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Dis3 
PIN domain
necessary binding region
aa 62-189
Dis3 
C3 domain
necessary binding region
aa 31-63
Dis3 
STAG domain
necessary binding region
aa 205-222
Dis3 
OB1 domain
necessary binding region
aa 253-312
Dis3 
OB domains
necessary binding region
aa 298-364
Dis3 
OB2 domain
necessary binding region
aa 365-438
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Graham et al., 2009)
FBrf0211792-1.coIP.WB
Reference
Mamolen et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Dis3 

dDis3

bait

FLAG tag

Dis3 
Dis3 
Rrp42 

dRrp42

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Dis3 
N-terminal domain
necessary binding region
aa 1-28
Dis3 
N-terminal domain
sufficient binding region
aa 1-252
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Mamolen et al., 2010)
FBrf0218395-6776.DPiM
Reference
Guruharsha et al., 2011
Description
physical association
Assay
Collection
DPiM-2
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rrp42 
unspecified role
Rrp42 
unspecified role
Rrp42 
unspecified role
Dis3 
unspecified role
Dis3 
unspecified role
Dis3 
unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

HGScore = 137.89530

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)