Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell extract.

Endogenous Abl binds specifically to Lar, but not to other protein tyrosine phosphatases tested, Ptp69D and Ptp10D.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in baculovirus system.

Purified Abl was allowed to autophosphorylate in the presence of labelled γ[32]P-ATP before testing for binding to Lar.

Enzyme expressed and purified from bacteria.

Enzyme target derived from chemical synthesis.

Each of the D1 and D2 PTP domains can bind phosphopeptides independently, but with slightly different sequence specificities..