Bait endogenous to nuclear extract.

Prey endogenous to nuclear extract.

Source was embryonic nuclear extract of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

ph-p, ph-d, Pc, Psc and Scm copurify as part of a large polycomb repressive (PRC1) complex, estimated to be 2-6 MDa in size (gel filtration).

Affinity purified ph-p containing complexes were fractionated by gel filtration to estimate complex size.

Source was embryonic nuclear extract of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

ph-p, ph-d, Pc, Psc and Scm copurify as part of a polycomb repressive (PRC1) complex

Nuclear extract was fractionated on a Biorex 70 column, then used for FLAG-affinity purification of ph-p containing complexes.

Source was embryonic nuclear extract of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

ph-p, ph-d, Pc, Psc and Scm copurify as part of a polycomb repressive (PRC1) complex

Nuclear extract was fractionated on a Biorex 70 column, then used for FLAG-affinity purification of Pc containing complexes.

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

The antibodies used against ph-p are described in DeCamillis et al. Genes and Development vol. 6 1992.

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

The antibodies used against ph-p are described in DeCamillis et al. Genes and Development vol. 6 1992.

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

The affinity purified ph-p-containing complex was subjected to additional gel filtration and heparin affinity fractionation.

Source was embryonic nuclear extract of transgenic fly line; bait produced from endogenous gene; prey produced from tagged transgenic construct.

Coimmunoprecipitation of tagged prey was determined by pulldown of a radiolabeled LexA DNA probe (that binds specifically to the LexA DNA binding domain).

ph-p, Pc, Psc and Sce form the PRC1 core complex.

Source was cell extract of baculovirus-infected Sf9 insect cell line; bait produced from transfected construct; prey produced from transfected construct.

Source was cell extract of baculovirus-infected insect cell line; bait produced from transfected construct; prey produced from transfected construct.

Positive control.

The BirA biotin ligase was coexpressed to biotinylate Pc bait in vivo, which was affinity purified using streptavidin beads and eluted by cleaving the TEV site between bait and tag.

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

The BirA biotin ligase was coexpressed to biotinylate Pc bait in vivo, which was affinity purified using streptavidin beads and eluted by cleaving the TEV site between the bait and BioTag

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

ph-p exists in Pc-bound and Pc-unbound pools.

Nuclear extract was partially purified by ammonium sulphate precipitation and heparin affinity chromatography before use in immunoprecipitation.

Positive control.

Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Positive control.

Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Positive control

Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Positive control

Positive control.

Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Positive control.

Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Interaction in vitro; bait and prey produced recombinant fusion proteins in baculovirus and insect cell system.

The PRC1 complex consists of Psc, Pc and Sce.