FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
crb, Patj
FlyBase ID
FBig0000095646
Interaction Network
Interactions Browser links
crb network
Patj network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0107610-3.PD.A
Reference
Bhat et al., 1999
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
crb 

CRB

bait

GST tag

crb 
Patj 

DLT

prey

T7 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
crb 
intracellular domain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation..

(Bhat et al., 1999)
FBrf0125447-1.coIP.WB
Reference
Klebes and Knust, 2000
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
crb 

Crb

bait

Myc tag

crb 
crb 
Patj 

Dlt

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
crb 
intracellular domain
sufficient binding region
crb 
intracellular domain
necessary binding region
ERLI

\'ΔERLI\'

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Klebes and Knust, 2000)
FBrf0147164-2.coIP.WB
Reference
Pellikka et al., 2002
Description
physical association
Assay
Collection
Source/Stage
organism / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Patj 

Dlt

bait
crb 

Crb

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adults of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Pellikka et al., 2002)
FBrf0152113-2.coIP.WB
Reference
Medina et al., 2002
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Patj 

Dlt

bait
crb 

Crb

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embyos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Medina et al., 2002)
FBrf0180375-5.PD.WB
Reference
Sotillos et al., 2004
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
crb 

Crb

bait

GST tag

crb 
crb 
Patj 

Patj

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
crb 
intracellular domain
sufficient binding region
RNKRATRGTYSPSAQEYCNPRLEMDNVLKPPPEERLI
crb 
intracellular domain
necessary binding region
ERLI

"ΔERLI" construct

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.

(Sotillos et al., 2004)
FBrf0217316-10.coIP.WB
Reference
Pocha et al., 2011
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Patj 

DPatj

bait
crb 

Crb

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult head lysate.

(Pocha et al., 2011)
FBrf0217316-2.coIP.WB
Reference
Pocha et al., 2011
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Patj 

DPatj

bait
crb 

Crb

prey

11 peptides recovered

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult head lysate.

(Pocha et al., 2011)
FBrf0228512-5.coIP.WB
Reference
Sen et al., 2015
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
crb 

Crb

bait

GFP tag

Patj 

PATJ

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Sen et al., 2015)
FBrf0228512-7.coIP.WB
Reference
Sen et al., 2015
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
crb 

Crb

bait
Patj 

PATJ

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Sen et al., 2015)
FBrf0239211-12.PLA
Reference
Portela et al., 2018
Description
physical association
Assay
Collection
Source/Stage
eye disc / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
crb 

Crb

bait
Patj 

Patj

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was eye discs of transgenic fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Portela et al., 2018)

Positive control.

(Portela et al., 2018)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (8)