FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Smyd4-2, HDAC3
FlyBase ID
FBig0000095764
Interaction Network
Interactions Browser links
Smyd4-2 network
HDAC3 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0205731-2.coIP.WB
Reference
Thompson and Travers, 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Smyd4-2 

dSmyd4

bait

FLAG tag

HDAC3 

dHDAC3

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait expressed in transfected cells.

(Thompson and Travers, 2008)

Prey expressed in transfected cells.

(Thompson and Travers, 2008)
FBrf0205731-4.PD.A
Reference
Thompson and Travers, 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Smyd4-2 

dSmyd4

bait

GST tag

Smyd4-2 
HDAC3 

dHDAC3

prey

[35]S label

HDAC3 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Smyd4-2 
MYND domain
sufficient binding region
aa 208-377
HDAC3 
N-terminal region
sufficient binding region
aa 1-155
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait expressed and purified from bacteria.

(Thompson and Travers, 2008)

Prey derived from in vitro translation.

(Thompson and Travers, 2008)

GST pull down.

(Thompson and Travers, 2008)
FBrf0205731-6.PD.WB
Reference
Thompson and Travers, 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Smyd4-2 

dSmyd4

bait

GST tag

Smyd4-2 
HDAC3 

dHDAC3

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Smyd4-2 
MYND domain
sufficient binding region
aa 208-377
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait expressed and purified from bacteria.

(Thompson and Travers, 2008)

Prey expressed and purified from bacteria.

(Thompson and Travers, 2008)

GST pull down.

(Thompson and Travers, 2008)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)