FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
tyf, pAbp
FlyBase ID
FBig0000095881
Interaction Network
Interactions Browser links
tyf network
pAbp network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0213059-1.coIP.WB
Reference
Lim et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
tyf 

TYF

bait

V5 tag

tyf 
pAbp 

PABP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
tyf 
region
unspecified role
aa 1-1167

\'TYF-N\'

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was S2 cell line transfected with a tagged tyf construct.

(Lim et al., 2011)
FBrf0213059-2.coIP.WB
Reference
Lim et al., 2011
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
tyf 

TYF

bait

V5 tag

tyf 
pAbp 

PABP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
tyf 
region
unspecified role
aa 1-1167

\'TYF-N\'

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult head extract from transgenic line carrying a tagged tyf construct.

(Lim et al., 2011)
FBrf0213059-3.PD.A
Reference
Lim et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pAbp 

PABP

bait

GST pull down

tyf 

TYF

prey

[35]S label

tyf 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
tyf 
region
unspecified role
aa 1-1167

\'TYF-N\'

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Cloned reagents; interaction in solution.

(Lim et al., 2011)
FBrf0221582-9.coIP.WB
Reference
Zhang et al., 2013
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
tyf 

TYF

bait

V5 tag

pAbp 

dPABP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

RNAi against Atx2 reduces the interaction of tyf with pAbp.

(Zhang et al., 2013)

Source was adult head of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Zhang et al., 2013)
FBrf0221600-1.coIP.MS
Reference
Lim and Allada, 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
tyf 

TYF

bait

FLAG tag

tyf 
pAbp 

PABP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
tyf 
C-terminal region
sufficient binding region
aa 1624-1911

\'TYF-C5\'

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Lim and Allada, 2013)
FBrf0221600-3.coIP.WB
Reference
Lim and Allada, 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
tyf 

TYF

bait

FLAG tag

tyf 
tyf 
pAbp 

PABP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
tyf 
C-terminal region
sufficient binding region
aa 1624-1911

\'TYF-C5\'

tyf 
C-terminal region
necessary binding region
aa 1624-1911

\'TYF-ΔC5\'

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Lim and Allada, 2013)

RNAi against Atx2 disrupts the interaction of tyf with pAbp.

(Lim and Allada, 2013)
FBrf0221600-5.coIP.WB
Reference
Lim and Allada, 2013
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
tyf 

TYF

bait

FLAG tag

tyf 
pAbp 

PABP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
tyf 
C-terminal region
necessary binding region
aa 1624-1911

\'TYF-ΔC5\'

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult head of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Lim and Allada, 2013)
FBrf0235227-5.coIP.WB
Reference
Lee et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
tyf 

tyf

bait
pAbp 

pAbp

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Lee et al., 2017)
FBrf0235227-8.coIP.WB
Reference
Lee et al., 2017
Description
physical association
Assay
Collection
Source/Stage
head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
tyf 

tyf

bait

FLAG tag

tyf 
pAbp 

pAbp

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
tyf 
interaction domain
necessary binding region
aa 1624-1911

coordinates relative to tyf-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Lee et al., 2017)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)