FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
CG12096, PI31
FlyBase ID
FBig0000096944
Interaction Network
Interactions Browser links
CG12096 network
PI31 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0221396-11.FW.A
Reference
Cho-Park and Steller, 2013
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
PI31 

PI31

bait

HMK tag, P[32] label

CG12096 

dS5b

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

In vitro interaction; bait produced as a recombinant fusion protein in bacterial system; prey produced in bacterial system (prey from embryo cDNA expression library).

(Cho-Park and Steller, 2013)

PI31 protein labeled by protein kinase A via the HMK tag, then used to screen a cDNA expression library.

(Cho-Park and Steller, 2013)
FBrf0221396-14.coIP.WB
Reference
Cho-Park and Steller, 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
CG12096 

dS5b

bait

FLAG tag

CG12096 
CG12096 
PI31 

DmPI31

prey

HA tag

PI31 
PI31 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
PI31 
C-terminal region
mutation disrupting interaction
aa 210aa 241

L210A

F241A

PI31 
HbYX domain
necessary binding region
CG12096 
motif I
mutation disrupting interaction
aa 44

E44

CG12096 
motif III
mutation disrupting interaction
aa 263

R263A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK293 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Cho-Park and Steller, 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)