egl, BicD Physical Interaction report

Collection

Source/Stage

ovary / adult stage female

Cell line used

Participants

Corresponds to

Reported as

Role

Note

egl

EGL

bait

egl

BicD

BICD

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

egl

N-terminal region

sufficient binding region

aa 35

C35Y, corresponds to egl[4e] allele

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was adult female ovary of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Mach and Lehmann, 1997)

FBrf0092619-2.coIP.WB

Reference

Mach and Lehmann, 1997

Description

physical association

Assay

Collection

Source/Stage

ovary / adult stage female

Cell line used

Participants

Corresponds to

Reported as

Role

Note

BicD

BICD

bait

egl

EGL

prey

egl

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

egl

N-terminal region

sufficient binding region

aa 35

C35Y, corresponds to egl[4e] allele

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was adult female ovary of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Mach and Lehmann, 1997)

FBrf0174997-6.coIP.WB

Reference

Navarro et al., 2004

Description

physical association

Assay

Collection

Source/Stage

ovary / adult stage female

Cell line used

Participants

Corresponds to

Reported as

Role

Note

BicD

bait

egl

Egl

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was adult female ovary of transgenic fly line; bait produced from from endogenous gene; prey produced from endogenous gene.

(Navarro et al., 2004)

FBrf0208220-1.Y2H

Reference

Dienstbier et al., 2009

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

egl

Egl

bait

fused to GAL4 DNA-binding domain

BicD

prey

fused to GAL4 activation domain

BicD

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

BicD

C-terminal domain

sufficient binding region

aa 647-782

\'CTD\'

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from cDNA expression library).

(Dienstbier et al., 2009)

44 BicD clones were recovered in a screen for egl-interacting factors.

(Dienstbier et al., 2009)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Dienstbier et al., 2009)

FBrf0208220-2.Y2H

Reference

Dienstbier et al., 2009

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

egl

Egl

bait

fused to GAL4 DNA-binding domain

egl

BicD

prey

fused to GAL4 activation domain

BicD

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

egl

N-terminal region

sufficient binding region

aa 1-257

BicD

C-terminal domain

sufficient binding region

aa 608-782

\'CTD\'

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Dienstbier et al., 2009)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Dienstbier et al., 2009)

FBrf0208220-3.Y2H

Reference

Dienstbier et al., 2009

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

BicD

bait

fused to GAL4 DNA-binding domain

BicD

egl

Egl

prey

fused to GAL4 activation domain

egl

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

egl

N-terminal region

sufficient binding region

aa 1-79

BicD

C-terminal domain

sufficient binding region

aa 536-782

\'CTD\'

BicD

coiled-coil domain

mutation disrupting interaction

aa 730

K730M

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Dienstbier et al., 2009)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Dienstbier et al., 2009)

FBrf0208220-6.PD.A

Reference

Dienstbier et al., 2009

Description

physical association

Assay

pull down

autoradiography

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

BicD

bait

GST tag

BicD

egl

Egl

prey

[35]S label

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

BicD

C-terminal domain

sufficient binding region

aa 536-782

\'CTD\'

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Dienstbier et al., 2009)

FBrf0208220-7.coIP.WB

Reference

Dienstbier et al., 2009

Description

physical association

Assay

Collection

Source/Stage

ovary / adult stage female

Cell line used

Participants

Corresponds to

Reported as

Role

Note

BicD

bait

GFP tag

BicD

egl

Egl

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

BicD

coiled-coil domain

mutation disrupting interaction

aa 730

K730M

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Dienstbier et al., 2009)

FBrf0211628-4.coIP.WB

Reference

Bianco et al., 2010

Description

physical association

Assay

Collection

Source/Stage

organism / embryonic stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

BicD

bait

egl

Egl

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Bianco et al., 2010)

FBrf0211628-5.coIP.WB

Reference

Bianco et al., 2010

Description

physical association

Assay

Collection

Source/Stage

organism / embryonic stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

egl

Egl

bait

GFP tag

BicD

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Bianco et al., 2010)

FBrf0214798-1.coIP.MS

Reference

Vazquez-Pianzola et al., 2011

Description

physical association

Assay

Identification by mass spectrometry

Collection

Source/Stage

organism / embryonic stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

BicD

bait

egl

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Vazquez-Pianzola et al., 2011)

FBrf0214798-3.coIP.WB

Reference

Vazquez-Pianzola et al., 2011

Description

physical association

Assay

Collection

Source/Stage

organism / embryonic stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

BicD

bait

egl

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Vazquez-Pianzola et al., 2011)

FBrf0221802-7.PD.A

Reference

Liu et al., 2013

Description

physical association

Assay

pull down

autoradiography

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

BicD

bait

His tag

BicD

egl

Egl

prey

[35]S label

egl

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

BicD

coiled-coil segment 3

sufficient binding region

aa 702-743

\'CC3\', coordinates relative to 782 aa isoform

BicD

coiled-coil segment 3

mutation disrupting interaction

aa 723aa 724aa 725aa 727aa 731

L723A

R724A

L725A

V727A

L731A

egl

N-terminal region

sufficient binding region

aa 1-79

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Purified recombinant proteins were tested for interaction.

(Liu et al., 2013)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Liu et al., 2013)

There is overlap of egl and Rab6 binding sites in BicD.

(Liu et al., 2013)

FBrf0221802-8.PD.WB

Reference

Liu et al., 2013

Description

physical association

Assay

pull down

anti tag western blot

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

egl

Egl

bait

GST tag

egl

BicD

prey

His tag

BicD

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

BicD

coiled-coil segment 3

sufficient binding region

aa 656-745

\'CC3\', coordinates relative to 782 aa isoform

egl

N-terminal region

sufficient binding region

aa 1-79

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Purified recombinant proteins were tested for interaction.

(Liu et al., 2013)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Liu et al., 2013)

Rab6 and egl compete for binding to BicD, as increasing amounts of GTP-bound Rab6 prevent binding of BicD to egl. There is overlap of egl and Rab6 binding sites in BicD.

(Liu et al., 2013)

FBrf0221802-9.coIP.WB

Reference

Liu et al., 2013

Description

physical association

Assay

Collection

Source/Stage

organism / embryonic stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

BicD

bait

GFP tag

BicD

egl

Egl

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

BicD

coiled-coil segment 3

mutation decreasing interaction

aa 731

L731A

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Liu et al., 2013)

FBrf0224776-2.coIP.MS

Reference

Vazquez-Pianzola et al., 2014

Description

physical association

Assay

peptide massfingerprinting

Collection

Source/Stage

organism / embryonic stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

BicD

Bic-D

bait

egl

Egl

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Vazquez-Pianzola et al., 2014)

FBrf0224776-4.coIP.WB

Reference

Vazquez-Pianzola et al., 2014

Description

physical association

Assay

Collection

Source/Stage

organism / embryonic stage ovary / female adult stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

BicD

Bic-D

bait

egl

Egl

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Positive control.

(Vazquez-Pianzola et al., 2014)

Source was embryos or adult female ovaries of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Vazquez-Pianzola et al., 2014)

FBrf0234579-12.coIP.SEQ

Reference

Vazquez-Pianzola et al., 2017

Description

physical association

Assay

full identification by DNA sequencing

Collection

Source/Stage

organism / embryonic stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

egl

Egl

bait

GFP tag

BicD

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Vazquez-Pianzola et al., 2017)

FBrf0234579-1a.coIP.WB

Reference

Vazquez-Pianzola et al., 2017

Description

physical association

Assay

Collection

Source/Stage

organism / embryonic stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

egl

Egl

bait

GFP tag

BicD

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Vazquez-Pianzola et al., 2017)

FBrf0239530-3.EM

Reference

Sladewski et al., 2018

Description

physical association

Assay

electron microscopy

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

BicD

neutral component

YFP tag

BicD

neutral component

YFP tag

BicD

neutral component

YFP tag

egl

Egl

neutral component

egl

Egl

neutral component

egl

Egl

neutral component

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; proteins produced as recombinant fusion proteins in baculovirus and Sf9 cell system.

(Sladewski et al., 2018)

FBrf0243139-18.coIP.WB

Reference

Goldman et al., 2019

Description

physical association

Assay

Collection

Source/Stage

ovary / female adult stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

BicD

bait

egl

Egl

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was ovaries of transgenic fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Goldman et al., 2019)

FBrf0243139-3.coIP.WB

Reference

Goldman et al., 2019

Description

physical association

Assay

Collection

Source/Stage

ovary / female adult stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

egl

Egl

bait

RFP tag

egl

BicD

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

egl

interaction domain residues 965,969

mutation decreasing interaction

aa 965,969

S965K,S969R, coordinates relative to egl-PB

egl

N-terminal domain residue 35

mutation decreasing interaction

aa 35

C35Y, coordinates relative to egl-PB

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Goldman et al., 2019)

FBrf0243139-4.coIP.WB

Reference

Goldman et al., 2019

Description

physical association

Assay

Collection

Source/Stage

Cell line used

S2-DRSC

Participants

Corresponds to

Reported as

Role

Note

egl

Egl

bait

RFP tag

egl

BicD

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

egl

interaction domain residues 965,969

mutation decreasing interaction

aa 965,969

S965K,S969R, coordinates relative to egl-PB

egl

N-terminal domain residue 35

mutation decreasing interaction

aa 35

C35Y, coordinates relative to egl-PB

egl

RNA binding domain

mutation decreasing interaction

unspecified

deletion of RNA binding domain

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Goldman et al., 2019)

The egl-BicD interaction is restored with a S965K,S969R egl mutant if egl is artificaially dimerized.

(Goldman et al., 2019)

FBrf0250118-1a.coIP.WB

Reference

Neiswender et al., 2021

Description

physical association

Assay

Collection

Source/Stage

ovary / female adult stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

egl

Egl

bait

GFP tag

egl

BicD

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

egl

2pt interaction domain residues 965 and 969

mutation decreasing interaction

aa 965 and 969

S965K and S969R; coordinates relative to egl-PB

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Endogenous egl was depleted with shRNA. Introduction of a yeast leucine zipper to restore dimerization of egl 2pt mutant restores interaction with BicD.

(Neiswender et al., 2021)

Source was ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Neiswender et al., 2021)

FBrf0251875-11.coIP.WB

Reference

Goldman et al., 2021

Description

physical association

Assay

Collection

Source/Stage

ovary / female adult stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

egl

Egl

bait

GFP tag

egl

BicD

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

egl

RNA binding domain residues 676-726

mutation decreasing interaction

nt 676-726

truncated RNA binding domain; coordinates relative to egl-PB

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was adult ovaries; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Goldman et al., 2021)

FBrf0258683-2.Y2H

Reference

Jejina et al., 2024

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

egl

Egl

bait

fused to GAL4 DNA-binding domain

BicD

prey

fused to GAL4 activation domain

BicD

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

BicD

C-terminal domain

sufficient binding region

unspecified

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD; positive control.

(Jejina et al., 2024)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Jejina et al., 2024)

FBrf0258683-3.Y2H

Reference

Jejina et al., 2024

Description

physical association

Assay