ci, rdx Physical Interaction report

western blot

Collection

Source/Stage

organism / embryonic stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

rdx

bait

MYC tag

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Kent et al., 2006)

FBrf0194555-1.coIP.WB

Reference

Zhang et al., 2006

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

rdx

HIB

bait

FLAG tag

rdx

prey

Myc tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

rdx

MATH domain

sufficient binding region

aa 1-179

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Zhang et al., 2006)

The interaction of full length rdx with ci is increased in the presence of proteasome inhibitor MG132.

(Zhang et al., 2006)

Su(fu) and rdx compete for binding to ci.

(Zhang et al., 2006)

FBrf0194555-2.coIP.WB

Reference

Zhang et al., 2006

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

bait

Myc tag

rdx

HIB

prey

FLAG tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

N-terminal region

sufficient binding region

aa 1-440

C-terminal region

sufficient binding region

aa 1160-1377

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Zhang et al., 2006)

Both the N- and C-terminal regions of ci bind rdx.

(Zhang et al., 2006)

FBrf0194555-3.PD.WB

Reference

Zhang et al., 2006

Description

physical association

Assay

pull down

western blot

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

rdx

HIB

bait

GST tag

rdx

prey

Myc tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

rdx

MATH domain

sufficient binding region

aa 1-179

N-terminal region

sufficient binding region

aa 1-440

C-terminal region

sufficient binding region

aa 1160-1377

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell line.

(Zhang et al., 2006)

FBrf0194555-4.Y2H

Reference

Zhang et al., 2006

Description

physical association

Assay

two hybrid

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

bait

fused to GAL4 DNA-binding domain

rdx

HIB

prey

fused to GAL4 activation domain

rdx

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

rdx

MATH domain

sufficient binding region

aa 1-179

N-terminal region

sufficient binding region

aa 1-440

C-terminal region

sufficient binding region

aa 1268-1377

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Zhang et al., 2006)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Zhang et al., 2006)

FBrf0212658-1.Y2H

Reference

Seong et al., 2010

Description

physical association

Assay

two hybrid

Collection

Source/Stage

organism / adult stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

bait

fused to LexA DNA-binding domain

rdx

prey

fused to GAL4 activation domain

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

N-terminal region

sufficient binding region

aa 1-441

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Two-hybrid system: yeast LexA-BD/GAL4-AD

(Seong et al., 2010)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from adult cDNA expression library).

(Seong et al., 2010)

FBrf0212658-4.coIP.WB

Reference

Seong et al., 2010

Description

physical association

Assay

Collection

Source/Stage

Cell line used

CME-W1-Cl.8+

Participants

Corresponds to

Reported as

Role

Note

bait

HA tag

rdx

prey

FLAG tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

rdx and Kap-α3 compete for binding to ci.

(Seong et al., 2010)

Source was cell extract of clone8 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Seong et al., 2010)

FBrf0215181-1.Y2H

Reference

Zhang et al., 2009

Description

physical association

Assay

two hybrid

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

bait

fused to GAL4 DNA-binding domain

rdx

HIB

prey

fused to GAL4 activation domain

rdx

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

interaction domain

sufficient binding region

aa 268-378

coordinates relative to ci-PA

interaction domain

necessary binding region

aa 369-378

coordinates relative to ci-PA

interaction domain

mutation disrupting interaction

aa 369aa 372aa 373aa 374aa 375

E369A, coordinates relative to ci-PA

S372A, coordinates relative to ci-PA

S373A or S373D, coordinates relative to ci-PA

S374A or S374D, coordinates relative to ci-PA

S375A or S375D, coordinates relative to ci-PA

rdx

N-terminal domain

sufficient binding region

aa 1-179

coordinates relative to rdx-PB

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Zhang et al., 2009)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Zhang et al., 2009)

FBrf0215181-2.PD.WB

Reference

Zhang et al., 2009

Description

physical association

Assay

pull down

western blot

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

bait

GST tag

rdx

HIB

prey

HA tag

rdx

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

rdx

N-terminal

sufficient binding region

aa 1-179

coordinates relative to rdx-PB

S,T rich domain 1

sufficient binding region

aa 46-57

coordinates relative to ci-PA

S,T rich domain 2

sufficient binding region

aa 216-227

coordinates relative to ci-PA, observed interaction is weak

S,T rich domain 3

sufficient binding region

aa 368-376

coordinates relative to ci-PA

S,T rich domain 4

sufficient binding region

aa 379-392

coordinates relative to ci-PA

S,T rich domain 5

sufficient binding region

aa 1267-1276

coordinates relative to ci-PA, observed interaction is weak

S,T rich domain 6

sufficient binding region

aa 1359-1366

coordinates relative to ci-PA

S,T rich domain 4

mutation increasing interaction

aa 386

A386S, coordinates relative to ci-PA

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell extract.

(Zhang et al., 2009)

Six S,T rich motifs in ci independently bind rdx.

(Zhang et al., 2009)

FBrf0215181-3.coIP.WB

Reference

Zhang et al., 2009

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

bait

Myc or HA tag

rdx

HIB

prey

FLAG or Myc tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

N-terminal domain

sufficient binding region

aa 1-440

coordinates relative to ci-PA

C-terminal domain

sufficient binding region

aa 1160-1377

coordinates relative to ci-PA

interaction domain

mutation decreasing interaction

aa 372-375,382,384-385,387-388,1363-1366aa 50-52,218-220,223,225,1268,1270,1273,1275

all S to A mutations,coordinates relative to ci-PA

coordinates relative to ci-PA

S,T rich domains 1-6

mutation disrupting interaction

50-52,218-220,223,225,372-375,382,384-385,387-388,1268,1270,1273,1275,1363-1366

all S to A mutations, coordinates relative to ci-PA

S,T rich domains 1-4,6,w

mutation disrupting interaction

50-52,218-220,223,225,372-375,382,384-385,387-388,1363-1366,1293-1295

S,T to A mutations in the context of full length ci, coordinates relative to ci-PA

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Zhang et al., 2009)

FBrf0215181-4.coIP.WB

Reference

Zhang et al., 2009

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

rdx

HIB

bait

FLAG tag

prey

Myc tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

N-terminal domain

sufficient binding region

aa 1-440

coordinates relative to ci-PA

N-terminal domain

necessary binding region

aa 1-267

in the context of N-terminal fragment, coordinates relative to ci-PA

interaction domain

sufficient binding region

aa 1160-1306

coordinates relative to ci-PA

S,T rich domains 1-6

mutation disrupting interaction

50-52,218-220,223,225,372-375,382,384-385,387-388,1268,1270,1273,1275,1363-1366

all S to A mutations, coordinates relative to ci-PA

S,T rich domains 3 and 4

mutation disrupting interaction

372-375,382,384-385,387-388

S to A mutations in the context of N-terminal fragment aa 1-440, coordinates relative to ci-PA

S,T rich domains 2 and 3

mutation decreasing interaction

218-220,223,225,372-375

S to A mutations in the context of N-terminal fragment aa 1-440, coordinates relative to ci-PA

S,T rich domains 2 and 4

mutation decreasing interaction

218-220,223,225,382,384-385,387-388

S to A mutations in the context of N-terminal fragment aa 1-440, coordinates relative to ci-PA

S,T rich domains 1-4

mutation decreasing interaction

50-52,218-220,223,225,372-375,382,384-385,387-388

S to A mutations in the context of full length ci, coordinates relative to ci-PA

S,T rich domains 1-5

mutation disrupting interaction

50-52,218-220,223,225,372-375,382,384-385,387-388,1268,1270,1273,1275

S to A mutations in the context of full length ci, coordinates relative to ci-PA

C-terminal domain

necessary binding region

aa 1288-1397

in the context of C-terminal fragment, aa 1160-1397, coordinates relative to ci-PA

S,T rich domains 6 and w

mutation disrupting interaction

1363-1366,1293-1295

S,T to A mutations in the context of C-terminal fragment aa 1160-1377, coordinates relative to ci-PA

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Zhang et al., 2009)

Mutation of ci S,T motifs 3,4 and 5 have the strongest effect on rdx binding.

(Zhang et al., 2009)

FBrf0221943-14a.ENZ.MS

Reference

Description

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

rdx

Rdx

enzyme

FLAG tag

rdx

Rdx

enzyme

FLAG tag

enzyme target

GST tag

enzyme target

GST tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

FBrf0224822-1.coIP.WB

Reference

Liu et al., 2014

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

CiN

bait

HA tag

rdx

HIB

prey

Myc tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Positive control.

(Liu et al., 2014)

No interaction detected between rdx and crn.

(Liu et al., 2014)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Liu et al., 2014)

FBrf0224822-2.coIP.WB

Reference

Liu et al., 2014

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

rdx

HIB

bait

Myc tag

CiN

prey

HA tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Positive control.

(Liu et al., 2014)

No interaction detected between rdx and crn.

(Liu et al., 2014)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Liu et al., 2014)

FBrf0228220-1.coIP.WB

Reference

Shi et al., 2014

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

bait

Myc tag

rdx

HIB

prey

HA tag

rdx

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

rdx

MATH domain

sufficient binding region

coordinates not reported

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Shi et al., 2014)

The ci-rdx interaction is reduced upon co-transfection of CK1 enzymes (CkIα, dco), and increased by CK1 dsRNA treatment. hh treatment stimulates the ci-rdx interaction.

(Shi et al., 2014)

FBrf0228220-3a.PD.WB

Reference

Shi et al., 2014

Description

physical association

Assay

pull down

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

bait

GST tag

rdx

HIB

prey

HA tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

HIB binding site S4

sufficient binding region

AQVEADSASSQLSD

HIB binding site S6

sufficient binding region

TLFPDVSSSTHPYHG

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected S2 cell extract.

(Shi et al., 2014)

FBrf0229673-8.coIP.WB

Reference

Zhou et al., 2015

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

rdx

Hib

bait

HA tag

prey

Myc tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

The rdx-ci interaction is not affected by cotransfection of Usp7.

(Zhou et al., 2015)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Zhou et al., 2015)

FBrf0245753-5.coIP.WB

Reference

Han et al., 2019

Description

physical association

Assay