FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
baz, Stat92E
FlyBase ID
FBig0000097457
Interaction Network
Interactions Browser links
baz network
Stat92E network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0204740-1.coIP.WB
Reference
Sotillos et al., 2008
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
baz 

Par-3

bait
Stat92E 

STAT

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; prey produced from tagged transgenic construct; bait produced from endogenous gene.

(Sotillos et al., 2008)
FBrf0204740-2.coIP.WB
Reference
Sotillos et al., 2008
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Stat92E 

STAT

bait

GFP tag

baz 

Par-3

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Sotillos et al., 2008)
FBrf0221056-1.coIP.WB
Reference
Sotillos et al., 2013
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Stat92E 

STAT

bait

GFP tag

Stat92E 
Stat92E 
baz 

Baz

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Stat92E 
C-terminal region
sufficient binding region
aa 340-761
Stat92E 
transactivation domain
necessary binding region
aa 659-761
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Sotillos et al., 2013)
FBrf0221056-2.coIP.WB
Reference
Sotillos et al., 2013
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
baz 

Baz

bait
Stat92E 

STAT

prey

GFP tag

Stat92E 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Stat92E 
C-terminal region
sufficient binding region
aa 340-761
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from endogenous gene; prey produced from tagged transgenic construct.

(Sotillos et al., 2013)
FBrf0221056-3.PD.WB
Reference
Sotillos et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
baz 

Baz

bait

GST tag

baz 
baz 
Stat92E 

STAT

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
baz 
N-terminal region
sufficient binding region
aa 1-317
baz 
C-terminal region
sufficient binding region
aa 1048-1434
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.

(Sotillos et al., 2013)

Stat92E can bind both the N- and C-terminal regions of baz.

(Sotillos et al., 2013)
FBrf0221056-4.PD.A
Reference
Sotillos et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
baz 

Baz

bait

GST tag

baz 
baz 
Stat92E 

STAT

prey

[35]S label

Stat92E 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
baz 
N-terminal region
sufficient binding region
aa 1-317
baz 
C-terminal region
sufficient binding region
aa 1048-1434
Stat92E 
C-terminal region
sufficient binding region
aa 340-761
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Sotillos et al., 2013)

Stat92E can bind both the N- and C-terminal regions of baz.

(Sotillos et al., 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)