Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

rols interacts with kirre and mbc, but not with sns or blow.

A cleaved form of kirre that most likely represents the transmembrane and cytosolic domains coimmunoprecipitates with rols. However, the cytoplasmic domain of kirre is alone not sufficient for rols binding.

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast LexA-BD/B42-AD