FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
IntS1, IntS12
FlyBase ID
FBig0000097557
Interaction Network
Interactions Browser links
IntS1 network
IntS12 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0220828-1.coIP.WB
Reference
Chen et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
IntS12 

IntS12

bait

FLAG tag

IntS12 
IntS12 
IntS12 
IntS1 

IntS1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
IntS12 
N-terminal microdomain
necessary binding region
aa 1-45
IntS12 
N-terminal microdomain
sufficient binding region
aa 1-45

this region less efficient than full length IntS12

IntS12 
N-terminal microdomain
mutation decreasing interaction
aa 26-30aa 36-40

LHSSN converted to Alanine residues

ELRLL converted to Alanine residues

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Chen et al., 2013)
FBrf0220828-3.Y2H
Reference
Chen et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
IntS1 

IntS1

bait

fused to GAL4 DNA-binding domain

IntS12 

IntS12

prey

fused to GAL4 activation domain

IntS12 
IntS12 
IntS12 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
IntS12 
N-terminal microdomain
necessary binding region
aa 1-45
IntS12 
N-terminal microdomain
sufficient binding region
aa 1-45

this region less efficient than full length IntS12

IntS12 
N-terminal microdomain
mutation decreasing interaction
aa 26-30aa 36-40

LHSSN converted to Alanine residues

ELRLL converted to Alanine residues

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Chen et al., 2013)

IntS12 binds IntS1, but not other Integrator subunits, in this assay.

(Chen et al., 2013)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Chen et al., 2013)
FBrf0247102-35.coIP.WB
Reference
Huang et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
IntS1 

IntS1

bait

FLAG tag

IntS12 

IntS12

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Huang et al., 2020)
FBrf0247102-5.coIP.MS
Reference
Huang et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
IntS1 

IntS1

bait

FLAG tag

IntS12 

IntS12

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Huang et al., 2020)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)