Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

All three "C2-WW-HECT" E3 family proteins can bind to the C-terminal domain of ptc : Su(dx), Nedd4 and Smurf.

Two-hybrid system: yeast GAL4-BD/GAL4-AD

Treatment of cells with hh conditioned medium or depletionof smo increased the ptc-Nedd4 interaction.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.