FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Pur-α, Fmr1
FlyBase ID
FBig0000097609
Interaction Network
Interactions Browser links
Pur-α network
Fmr1 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0219614-8.coIP.WB
Reference
Aumiller et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Pur-α 

Pur-α

bait

GFP tag

Pur-α 
Pur-α 
Fmr1 

FMRP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Pur-α 
PUR repeats I and II
sufficient binding region
aa 40-185
Pur-α 
PUR repeats I and II
mutation disrupting interaction
aa 80-158

R80A, R158A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Aumiller et al., 2012)

Mutation of the Pur-α RNA binding domain disrupts interaction with Fmr1 protein.

(Aumiller et al., 2012)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)