FB2024_03 , released June 25, 2024
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
IntS12, IntS9
FlyBase ID
FBig0000097803
Interaction Network
Interactions Browser links
IntS12 network
IntS9 network
esyN Network Diagram
Show neighbor-neighbor interactions:
Select Layout:
Legend:
Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0220828-2.coIP.WB
Reference
Chen et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
IntS12 

IntS12

bait

FLAG tag

IntS12 
IntS12 
IntS12 
IntS9 

IntS9

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
IntS12 
N-terminal microdomain
necessary binding region
aa 1-45
IntS12 
N-terminal microdomain
sufficient binding region
aa 1-45

this region less efficient than full length IntS12

IntS12 
N-terminal microdomain
mutation decreasing interaction
aa 26-30aa 36-40

LHSSN converted to Alanine residues

ELRLL converted to Alanine residues

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Chen et al., 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)