FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
N, spdo
FlyBase ID
FBig0000097823
Interaction Network
Interactions Browser links
N network
spdo network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0162056-1.coIP.WB
Reference
O'Connor-Giles and Skeath, 2003
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
N 

Notch

bait
spdo 

Spdo

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(O'Connor-Giles and Skeath, 2003)
FBrf0221478-2.PD.WB
Reference
Upadhyay et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
N 

NICD

bait

His, FLAG tag

N 
spdo 

Sanpodo

prey

FLAG tag

spdo 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
spdo 
amino terminal cytoplasmic region
sufficient binding region
aa 100-424

\'ACTR\'

N 
intracellular domain
sufficient binding region

110 kDa

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Upadhyay et al., 2013)
FBrf0221478-3.coIP.WB
Reference
Upadhyay et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
N 

NICD

bait
spdo 

Sanpodo

prey

FLAG tag

spdo 
spdo 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
spdo 
amino terminal cytoplasmic region
sufficient binding region
aa 1-424
spdo 
N-terminal region
necessary binding region
aa 1-189
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Upadhyay et al., 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)