FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
egl
FlyBase ID
FBig0000098052
Interaction Network
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egl network
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0174997-7.coIP.WB
Reference
Navarro et al., 2004
Description
physical association
Assay
Collection
Source/Stage
ovary / adult stagefemale
Cell line used
Participants
Corresponds to
Reported as
Role
Note
egl 

Egl

bait

Myc tag

egl 

Egl

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult female ovary of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Navarro et al., 2004)

Endogenous egl coimmunoprecipitates with Myc-tagged egl.

(Navarro et al., 2004)
FBrf0234579-25.coIP.SEQ
Reference
Vazquez-Pianzola et al., 2017
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
egl 

Egl

bait

GFP tag

egl 

egl

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Vazquez-Pianzola et al., 2017)
FBrf0234579-58a.coIP.PCR
Reference
Vazquez-Pianzola et al., 2017
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
egl 

Egl

bait

GFP tag

egl 

egl

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Vazquez-Pianzola et al., 2017)
FBrf0243139-16.coIP.WB
Reference
Goldman et al., 2019
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
egl 

Egl

bait

RFP tag

egl 

Egl

prey
egl 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
egl 
interaction domain residues 965,969
mutation decreasing interaction
aa 965,969

S965K,S969R, coordinates relative to egl-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Goldman et al., 2019)
FBrf0243139-17.coIP.WB
Reference
Goldman et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DRSC
Participants
Corresponds to
Reported as
Role
Note
egl 

Egl

bait

GFP tag

egl 

Egl

prey

FLAG tag

egl 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
egl 
interaction domain residues 965,969
mutation disrupting interaction
aa 965,969

S965K,S969R, coordinates relative to egl-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Goldman et al., 2019)
External Crossreferences and Linkouts ( 0 )
References (3)