FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000098123
Interaction Network
Interactions Browser links
tral network
cup network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0190228-1.coIP.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
tral 

Tral

bait
cup 

Cup

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

FBrf0190228-5.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
tral 

Tral

bait
cup 

cup

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult female ovaries of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

The tral-cup interaction is RNA-independent (resistant to RNase treatment).

FBrf0206982-3.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cup 

CUP

bait

HA tag

tral 

Tral

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

FBrf0206982-9.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
tral 

Tral

bait

HA tag

tral 
tral 
cup 

CUP

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
tral 
LSm domain
sufficient binding region
aa 1-96

coordinates relative to tral-PA

tral 
LSm domain residue 21
mutation decreasing interaction
aa 21

R21E, coordinates relative to tral-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0216681-8.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cup 

CUP

bait

HA tag

cup 
tral 

Tral

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
cup 
region
sufficient binding region
aa 417–770

\'Mid\' region; reduced compared to binding with full-length polypeptide

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

In the presence of RNase during immunoprecipitation this interaction is still observed, indicating that it is not mediated through RNA in the RNP complex; may be direct.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)