FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
mid, gro
FlyBase ID
FBig0000098205
Interaction Network
Interactions Browser links
mid network
gro network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0219199-1.coIP.WB
Reference
Formaz-Preston et al., 2012
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mid 

mid

bait

FLAG tag

mid 
gro 

Gro

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mid 
engrailed-homology-1 motif
mutation decreasing interaction
aa 41

F41E

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Formaz-Preston et al., 2012)
FBrf0219199-2.coIP.WB
Reference
Formaz-Preston et al., 2012
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
gro 

Gro

bait
mid 

Mid

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Formaz-Preston et al., 2012)
FBrf0219199-4.PD.A
Reference
Formaz-Preston et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mid 

Mid

bait

GST tag

mid 
mid 
gro 

Gro

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mid 
N-terminal region and T-box
sufficient binding region
aa 1-364
mid 
engrailed-homology-1 motif
mutation decreasing interaction
aa 41

F41E

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Formaz-Preston et al., 2012)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)