FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Past1, numb
FlyBase ID
FBig0000098332
Interaction Network
Interactions Browser links
Past1 network
numb network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0179447-1.PD.TV
Reference
Smith et al., 2004
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 17blastoderm stagegastrula stageembryonic stage 8dorsal closure stageembryonic stage 9embryonic stage 6early extended germ band stageembryonic cycle 3extended germ band stageembryonic stage 4embryonic cycle 9embryonic stage 2late extended germ band stageembryonic stage 5embryonic stage 11embryonic stage 3embryonic stage 12embryonic stage 15embryonic cycle 6embryonic cycle 5embryonic cycle 8embryonic cycle 7embryonic stage 13embryonic cycle 4embryonic stage 14embryonic stage 10embryonic stage 7embryonic stage 16
Cell line used
Participants
Corresponds to
Reported as
Role
Note
numb 

dNumb

bait

biotin tag

numb 
Past1 

dEHD

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
numb 
DPF and NPF motifs
sufficient binding region
ARHSTNPFISP
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced by chemical synthesis; prey produced as a recombinant fusion protein in bacteriophage system (prey from embryo cDNA expression library)..

(Smith et al., 2004)
FBrf0179447-2.PD.WB
Reference
Smith et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Past1 

dEHD

bait

GST tag

Past1 
numb 

dNumb

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Past1 
EH domain
sufficient binding region
aa 436-531
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from HEK293T cell extract.

(Smith et al., 2004)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)