Two-hybrid system: yeast LexA-BD/B42-AD
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
EGF repeats 11-14
EGF repeats 22-27
Interaction in vitro; bait purified from media of transfected S2 cells; prey purified from media of transfected S2 cells.
Interaction in vitro; protein produced as a recombinant fusion protein in a baculovirus and Sf9 cell system.
Molecular weight determined is consistent with a dimer.
