FB2024_03 , released April 23, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
gw, mir-ban
FlyBase ID
FBig0000098611
Interaction Network
Interactions Browser links
gw network
mir-ban network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0208025-2.coIP.NB
Reference
Eulalio et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
gw 

GW182

bait

HA tag

mir-ban 

Bantam

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Eulalio et al., 2009)

RRM domain of gw not necessary for gw-ban interaction.

(Eulalio et al., 2009)
FBrf0215100-5.coIP.NB
Reference
Zekri et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
gw 

GW182

bait

HA tag

gw 
mir-ban 

Bantam

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
gw 
GW repeats
mutation disrupting interaction

all N-terminal GW repeats mutated to Alanine

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Zekri et al., 2009)
FBrf0215456-9.coIP.NB
Reference
Miyoshi et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
gw 

GW182

bait
mir-ban 

bantam

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Anti-gw immunoprecipitates mature ban miRNA almost exclusively, and not ban pre-miRNA.

(Miyoshi et al., 2009)

Anti-gw immunoprecipitates are able to cleave target ban mRNA in vitro.

(Miyoshi et al., 2009)

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Miyoshi et al., 2009)
FBrf0215487-4.coIP.NB
Reference
Eulalio et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
gw 

GW182

bait

HA tag

gw 
mir-ban 

Bantam

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
gw 
interaction domain
mutation disrupting interaction
unspecified

all 12 N-terminal GW-repeats substituted with alanines, coordinates relative to gw-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Eulalio et al., 2009)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)