Two-hybrid system: yeast LexA-BD/B42-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

robo interacts with dock, but not Pak, Rac1, Rac2 or Mtl in this assay.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected HEK293T cell extract.

robo can bind ena and dock without any apparent competition between the two.

Cells were treated with the robo ligand sli for 10 minutes before the pull down assay.

Source was cell extract of HEK293T cell line; bait produced from transfected construct; prey produced from transfected construct.

sli stimulates the interaction of robo with dock.