CaMKII was incubated with [125]I labeled bovine calmodulin. Unlabeled recombinant Cam was added in 100-fold excess, and assayed for disruption of the coimmunoprecipitation of CaMKII with labeled bovine calmodulin.

Cam stimulates the in vitro kinase activity of CaMKII.

Interaction in vitro; bait derived from transfected COS cell extract; prey as a recombinant fusion protein in bacterial system.

13 CaMKII clones recovered in this phage display screen for Cam binding proteins.

Interaction in vitro; bait obtained from commercial source; prey produced as transgenic fusion protein in bacteriophage (prey from adult retina cDNA expression library).