FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
mof, mle
FlyBase ID
FBig0000099141
Interaction Network
Interactions Browser links
mof network
mle network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0129699-1.coIP.WB
Reference
Akhtar et al., 2000
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Schneider's-line-2
Participants
Corresponds to
Reported as
Role
Note
mle 

MLE

bait
mof 

MOF

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

This interaction persists upon RNase treatment.

(Akhtar et al., 2000)

Source was cell extract of SL2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Akhtar et al., 2000)
FBrf0129699-3.coIP.WB
Reference
Akhtar et al., 2000
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Schneider's-line-2
Participants
Corresponds to
Reported as
Role
Note
mof 

MOF

bait
mle 

MLE

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

This interaction persists upon RNase treatment.

(Akhtar et al., 2000)

The interaction of mle with mof is disrupted by high salt. By contrast, msl-2 and msl-3 still coimmunoprecipitate with mof under high salt conditions.

(Akhtar et al., 2000)

Source was cell extract of SL2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Akhtar et al., 2000)
FBrf0160428-10.coIP.WB
Reference
Buscaino et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
mle 

MLE

bait
mof 

MOF

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was whole cell extracts; bait produced from endogenous gene; prey produced from endogenous gene.

(Buscaino et al., 2003)
FBrf0229405-3.PLA
Reference
Lindehell et al., 2015
Description
physical association
Assay
Collection
Source/Stage
embryonic/larval salivary gland / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mle 

mle

neutral component
mle 

mle

neutral component
mle 

mle

neutral component
mof 

mof

neutral component
mof 

mof

neutral component
mof 

mof

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Factors were detected on polytene chromosomes using antibodies (to proteins) or biotinylated antisense probes (to ncRNA). The PLA signal develops only if probes to the two factors are in close proximity to each other (within tens of nanometers).

(Lindehell et al., 2015)

Source was larval salivary gland of wild-type fly line; proteins produced from endogenous gene.

(Lindehell et al., 2015)
FBrf0235529-4.coIP.WB
Reference
Schunter et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
mof 

mof

bait

GFP tag

mle 

mle

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Schunter et al., 2017)
FBrf0249283-2.coIP.WB
Reference
Aleman et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
mof 

MOF

bait
mle 

MLE

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Positive control.

(Aleman et al., 2021)

Source was nuclear extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Aleman et al., 2021)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (5)