FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
csw, msk
FlyBase ID
FBig0000099145
Interaction Network
Interactions Browser links
csw network
msk network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0134569-1.Y2H
Reference
Lorenzen et al., 2001
Description
physical association
Assay
Collection
Source/Stage
ovary / adult stagefemale
Cell line used
Participants
Corresponds to
Reported as
Role
Note
csw 

CSW

bait

fused to LexA DNA-binding domain

csw 
msk 

DIM-7

prey

fused to B42 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
csw 
C-terminal region
sufficient binding region
aa 655-841
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA-BD/B42-AD

(Lorenzen et al., 2001)

30% of csw-interacting clones represented msk cDNAs.

(Lorenzen et al., 2001)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from ovary cDNA expression library).

(Lorenzen et al., 2001)
FBrf0134569-2.Y2H
Reference
Lorenzen et al., 2001
Description
physical association
Assay
Collection
Source/Stage
imaginal disc / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
csw 

CSW

bait

fused to LexA DNA-binding domain

csw 
msk 

DIM-7

prey

fused to B42 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
csw 
C-terminal region
sufficient binding region
aa 655-841
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA-BD/B42-AD

(Lorenzen et al., 2001)

63% of csw-interacting clones represented msk cDNAs.

(Lorenzen et al., 2001)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from imaginal disc cDNA expression library).

(Lorenzen et al., 2001)
FBrf0134569-3.coIP.WB
Reference
Lorenzen et al., 2001
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
csw 

CSW

bait
msk 

DIM-7

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Lorenzen et al., 2001)
FBrf0134569-4.PD.WB
Reference
Lorenzen et al., 2001
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 10blastoderm stagegastrula stageembryonic stage 8embryonic stage 6embryonic stage 9embryonic cycle 3embryonic stage 4embryonic stage 2embryonic cycle 9embryonic stage 5embryonic stage 3embryonic cycle 6embryonic cycle 5embryonic cycle 8embryonic cycle 7embryonic cycle 4embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
csw 

CSW

bait

GST tag

csw 
msk 

DIM-7

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
csw 
PTP domain and C-terminal region
sufficient binding region
aa 486-841
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.

(Lorenzen et al., 2001)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)