CaMKII was immunoprecipitated, separated by SDS-PAGE and western blot were probed with both CaMKII and smt3 (SUMO) antibodies. N-ethylmalemide was added to inhibit SUMO-specific isopeptidases.
CaMKII is covalently modified by SUMO.
Source was adult heads of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.