FB2024_03 , released June 25, 2024
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
morgue, Ubi-p63E
FlyBase ID
FBig0000099218
Interaction Network
Interactions Browser links
morgue network
Ubi-p63E network
esyN Network Diagram
Show neighbor-neighbor interactions:
Select Layout:
Legend:
Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0222885-2.coIP.MS
Reference
Zhou et al., 2013
Description
physical association
Assay
Collection
Source/Stage
organism / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
morgue 

morgue

bait

FLAG tag

Ubi-p63E 

Ubiquitin

prey
Ubi-p63E 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The specific ubiquitin linkages were also determined by mass spectrometry.

(Zhou et al., 2013)

Source was adults of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Zhou et al., 2013)

morgue specifically associates with K48-linked ubiquitin multimers.

(Zhou et al., 2013)
FBrf0222885-5.coIP.WB
Reference
Zhou et al., 2013
Description
physical association
Assay
Collection
Source/Stage
organism / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
morgue 

morgue

bait

FLAG tag

Ubi-p63E 

Ubiquitin

prey
Ubi-p63E 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adults of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Zhou et al., 2013)

morgue specifically associates with ubiquitin multimers.

(Zhou et al., 2013)
FBrf0222885-8.PD.WB
Reference
Zhou et al., 2013
Description
physical association
Assay
Collection
Source/Stage
organism / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
morgue 

morgue

bait

MBP tag

morgue 
morgue 
Ubi-p63E 

Ubiquitin

prey
Ubi-p63E 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
morgue 
N-terminal region
sufficient binding region
aa 1-301
morgue 
C-terminal region
sufficient binding region
aa 302-491
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adults of transgenic fly line; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein and polymerized in vitro.

(Zhou et al., 2013)

Both the N-terminal and C-terminal regions of morgue bind polyubiquitin.

(Zhou et al., 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)