FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
CaMKII, CASK
FlyBase ID
FBig0000099363
Interaction Network
Interactions Browser links
CaMKII network
CASK network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0167958-1.coIP.WB
Reference
Lu et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
CaMKII 

CaMKII

bait

HA tag

CASK 

Cmg

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was transfected tsA201; bait produced from transfected construct; prey produced from transfected construct.

(Lu et al., 2003)
FBrf0167958-2.coIP.WB
Reference
Lu et al., 2003
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
CASK 

Cmg

bait
CaMKII 

CaMKII

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Lu et al., 2003)
FBrf0167958-3.PD.WB
Reference
Lu et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
CASK 

Cmg

bait

GST tag

CaMKII 

CaMKII

prey
CaMKII 
CaMKII 
CaMKII 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
CaMKII 
kinase domain
mutation increasing interaction
aa 43

K43M, kinase dead

CaMKII 
autophosphorylation site
mutation disrupting interaction
aa 306-307

T306D and T307D, mimics phosphorylated state

CaMKII 
autophosphorylation site
mutation increasing interaction
aa 306-307

T306A and T307A, mimics unphosphorylated state

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

CaMKII was pre-loaded with nucleotides in the presence of Ca[2+]/Cam before testing binding to CASK.

(Lu et al., 2003)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; produced as a recombinant fusion protein in baculovirus-infected Sf21 cell system.

(Lu et al., 2003)

CaMKII can bind to CASK in the absence of nucleotides, or when preloaded with ADP or AMP-PNP (a non-hydrolyzable ATP analog). However, pre-loading CaMKII with ATP inhibits binding to CASK. The interaction of CASK with kinase dead CaMKII is unaffected by nucleotide status.

(Lu et al., 2003)

Autophosphorylation of CaMKII disrupts its binding to CASK and to Cam.

(Lu et al., 2003)

CASK inhibits CaMKII activity in vitro.

(Lu et al., 2003)

Cam protects the CaMKII-CASK complex from dissociation upon the addition of ATP.

(Lu et al., 2003)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)