Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.
Source was cell extract of baculovirus-infected insect cell line; bait produced from transfected construct; prey produced from transfected construct.
Positive control.
Interaction in vitro; bait and prey produced as recombinant fusion protein in baculovirus and insect cell system.
Interaction in vitro; bait and prey produced as recombinant fusion protein in baculovirus and insect cell system.
Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Positive control.
Source was cell extract of baculovirus-infected insect cell line; bait produced from transfected construct; prey produced from transfected construct.
Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Nuclear extract was partially purified by ammonium sulphate precipitation and heparin affinity chromatography, then depleted of Pc-containing complexes before use in immunoprecipitation.
Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Nuclear extract was partially purified by ammonium sulphate precipitation and heparin affinity chromatography, then depleted of Pc-containing complexes before use in immunoprecipitation.
Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Positive control
Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Positive control.
Source was nuclear extract of recombinant baculovirus infected Sf9 cells; bait produced from transfected construct; prey produced from transfected construct.
Interaction in vitro; proteins co-produced as recombinant fusion proteins in a baculovirus and Sf9 cell system.
Interaction in vitro; proteins co-produced as recombinant fusion proteins in a baculovirus and Sf9 cell system.
Interaction in vitro; bait and prey produced recombinant fusion proteins in baculovirus and insect cell system.
