FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Topors, su(Hw)
FlyBase ID
FBig0000100561
Interaction Network
Interactions Browser links
Topors network
su(Hw) network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0191177-3.Y2H
Reference
Capelson and Corces, 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
su(Hw) 

Su(Hw)

bait

fused to LexA DNA-binding domain

Topors 

dTopors

prey

fused to VP16 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA-BD/VP16-AD

(Capelson and Corces, 2005)

Topors interacts with mod(mdg4) and su(Hw), but not Cp190 in this assay.

(Capelson and Corces, 2005)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Capelson and Corces, 2005)
FBrf0191177-5.PD.WB
Reference
Capelson and Corces, 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Topors 

dTopors

bait

GST tag

su(Hw) 

Su(Hw)

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Capelson and Corces, 2005)
FBrf0191177-8.coIP.WB
Reference
Capelson and Corces, 2005
Description
physical association
Assay
Collection
Source/Stage
adult head / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Topors 

dTopors

bait
su(Hw) 

Su(Hw)

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The interaction of Topors with mod(mdg4) is stronger than with su(Hw) or Cp190.

(Capelson and Corces, 2005)

Source was larvae of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Capelson and Corces, 2005)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)