FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
NT1, Toll-7
FlyBase ID
FBig0000100877
Interaction Network
Interactions Browser links
NT1 network
Toll-7 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0222474-10.coIP.WB
Reference
McIlroy et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
NT1 

DNT1

bait

V5 tag

Toll-7 

Toll-7

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(McIlroy et al., 2013)
FBrf0222474-12.coIP.WB
Reference
McIlroy et al., 2013
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
NT1 

DNT1

bait

FLAG tag

Toll-7 

Toll-7

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

(McIlroy et al., 2013)
FBrf0222474-6.ELISA
Reference
McIlroy et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
NT1 

DNT1

bait

V5 tag

Toll-7 

Toll-7

prey

HA tag

Toll-7 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Toll-7 
extracellular domain
sufficient binding region
aa 1-1047
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(McIlroy et al., 2013)
FBrf0222474-8.coIP.WB
Reference
McIlroy et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Toll-7 

Toll-7

bait

HA tag

NT1 

DNT1

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(McIlroy et al., 2013)
FBrf0242805-11.coIP.WB
Reference
Chowdhury et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-ATCC
Participants
Corresponds to
Reported as
Role
Note
NT1 

Spz-2

bait

FLAG tag

Toll-7 

Toll-7

prey

V5 tag

Toll-7 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Toll-7 
ecto domain
sufficient binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Chowdhury et al., 2019)
FBrf0242805-5.coIP.WB
Reference
Chowdhury et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-ATCC
Participants
Corresponds to
Reported as
Role
Note
Toll-7 

Toll-7

bait

V5 tag

Toll-7 
NT1 

Spz-2

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Toll-7 
ecto domain
sufficient binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Chowdhury et al., 2019)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)