Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected COS-7 cell extract.

Pak interacts specifically with GTP-bound Rac2, but not the GDP-bound form.

Pak interacts with Rac1 and Rac2, but not mtl.

GST-tagged Rac was preloaded with GDP or non-hydrolysable GTPγS.

Two-hybrid system: yeast LexA-BD/B42-AD

Pak interacts with Rac1 and Rac2 but not Mtl in this assay.

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

The Pak-Rac2 interaction is increased when cells express a functional robo receptor and are treated with the robo ligand sli.

Cells were treated with the robo ligand sli for 10 minutes before the pull down assay.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected HEK293T cell extract.