T5
[35]S label
DA
Interaction in vitro; bait produced and labeled by in vitro translation; prey produced and labeled by in vitro translation.
The da-l(1)sc interaction is stronger than da-ac, which is stronger than da-sc.
ac
GST tag
da
Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.
The E(spl)m2 site is weakly bound by da and not bound at all by ac but is bound strongly in the presence of both ac and da.
