FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Cdc7, chif
FlyBase ID
FBig0000102164
Interaction Network
Interactions Browser links
Cdc7 network
chif network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0227432-2.coIP.WB
Reference
Stephenson et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
chif 

Chiffon

bait

HA tag

chif 
Cdc7 

Cdc7

prey

His tag, FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
chif 
N-terminal region
sufficient binding region
aa 1-400
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of baculovirus-infected Sf21 insect cell line; bait produced from transfected construct; prey produced from transfected construct.

(Stephenson et al., 2015)

chif and l(1)G1048 were coexpressed in Sf21 cells, and purified by sequential nickel-agarose (His tag) pull down and HA-agarose affinity chromatography.

(Stephenson et al., 2015)
FBrf0227432-3.PD.WB
Reference
Stephenson et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cdc7 

Cdc7

bait

His tag, FLAG tag

chif 

Chiffon

prey

HA tag

chif 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
chif 
N-terminal region
sufficient binding region
aa 1-400
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of baculovirus-infected Sf21 insect cell line; bait produced from transfected construct; prey produced from transfected construct.

(Stephenson et al., 2015)

chif and l(1)G1048 were coexpressed in Sf21 cells, and purified by sequential nickel-agarose (His tag) pull down and HA-agarose affinity chromatography.

(Stephenson et al., 2015)
FBrf0227432-6.ENZ
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cdc7 

Cdc7

enzyme

His tag, FLAG tag

Cdc7 

Cdc7

enzyme

His tag, FLAG tag

chif 

Chiffon

stimulator

HA tag

chif 

Chiffon

stimulator

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0241207-18.coIP.MP
Reference
Torres-Zelada et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
chif 

Chiffon

bait

FLAG, HA tag

Cdc7 

Cdc7

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Torres-Zelada et al., 2019)
FBrf0241207-19.coIP.MP
Reference
Torres-Zelada et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Cdc7 

Cdc7

bait

FLAG, HA tag

chif 

Chiffon

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Torres-Zelada et al., 2019)
FBrf0241207-3.Y2H
Reference
Torres-Zelada et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
chif 

Chiffon

bait

fused to GAL4 DNA-binding domain

chif 
Cdc7 

Cdc7

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
chif 
N-terminal domain
sufficient binding region
aa 1-400

coordinates relative to chif-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Torres-Zelada et al., 2019)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Torres-Zelada et al., 2019)
FBrf0241207-4.Y2H
Reference
Torres-Zelada et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cdc7 

Cdc7

bait

fused to GAL4 DNA-binding domain

chif 

Chiffon

prey

fused to GAL4 activation domain

chif 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
chif 
N-terminal domain
sufficient binding region
aa 1-400

coordinates relative to chif-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Torres-Zelada et al., 2019)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Torres-Zelada et al., 2019)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)