FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
ben, nopo
FlyBase ID
FBig0000102224
Interaction Network
Interactions Browser links
ben network
nopo network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0206570-2.Y2H
Reference
Merkle et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ben 

BEN

bait

fused to GAL4 DNA-binding domain

ben 
nopo 

NOPO

prey

fused to GAL4 activation domain

nopo 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nopo 
RING domain
mutation disrupting interaction
aa 11

E11K, corresponds to nopo[Z1447] allele

ben 
interaction domain
mutation disrupting interaction
aa 97

P97S, corresponds to ben[1] allele

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Merkle et al., 2009)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Merkle et al., 2009)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)