FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Taf4, Taf6
FlyBase ID
FBig0000102547
Interaction Network
Interactions Browser links
Taf4 network
Taf6 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0192616-13.coIP.WB
Reference
Wright et al., 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Taf6 

TAF6

bait

FLAG tag

Taf6 
Taf4 

TAF4

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Taf6 
NTR domain
sufficient binding region
aa 1-227
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from tagged transfected construct; prey produced from endogenous gene.

(Wright et al., 2006)
FBrf0192616-3.coIP.WB
Reference
Wright et al., 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Taf4 

TAF4

bait
Taf6 

TAF6

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Wright et al., 2006)
FBrf0192616-8.coIP.WB
Reference
Wright et al., 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Taf4 

TAF4

bait

FLAG tag

Taf4 
Taf6 

TAF6

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Taf4 
CTR domain
sufficient binding region
aa 623-921

coordinates relative to Taf4-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from tagged transfected construct; prey produced from endogenous gene.

(Wright et al., 2006)
FBrf0209543-94.coIP.MS
Reference
Weake et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Taf6 

TAF6

bait

FLAG tag, HA tag

Taf4 

TAF4

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Weake et al., 2009)

The Drosophila TFIID complex is composed of the following subunits: e(y)1, (Taf9), Taf10b, Taf12, Taf1, Taf2, bip2, Taf4, Taf5, Taf6, Taf7, Taf8, Taf10, Taf11, Taf13, Tbp, Trf and Trf2@.

(Weake et al., 2009)

In Drosophila, Taf6 is restricted to the TFIID complex and is not found in the Drosophila SAGA complex.

(Weake et al., 2009)

Tandem affinity purification of bait and identification of prey by MudPIT.

(Weake et al., 2009)

Positive control.

(Weake et al., 2009)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)