Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein.
Two-hybrid system: yeast GAL4-BD/GAL4-AD, "In the case of the complete ARI-1 sequence, an autonomous transcription activity was detected that must be due to the acid-rich N-terminal sequences since this activity was absent in the ARI-1 aa48–503 fragment."
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
