FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
H, CtBP
FlyBase ID
FBig0000103524
Interaction Network
Interactions Browser links
H network
CtBP network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0136838-3.Y2H
Reference
Morel et al., 2001
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
H 

H

bait

fused to GAL4 DNA-binding domain

H 
H 
CtBP 

dCtBP

prey

fused to VP16 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
H 
dCtBP binding domain
necessary binding region
aa 1062-1076
H 
dCtBP binding domain
sufficient binding region
aa 1052-1076
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Morel et al., 2001)

Two-hybrid system: yeast GAL4-BD/VP16-AD

(Morel et al., 2001)
FBrf0136838-4.PD.A
Reference
Morel et al., 2001
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
CtBP 

dCtBP

bait

GST tag

H 

H

prey

[35]S label

H 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
H 
C-terminal region
necessary binding region
aa 711-1076
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Morel et al., 2001)
FBrf0151327-3.PD.A
Reference
Barolo et al., 2002
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
CtBP 

dCtBP

bait

GST tag

H 

H

prey

biotin label

H 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
H 
CtBP interaction domain
sufficient binding region
FHTSSLRNEQSSDLPLNLSKH
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey chemically synthesized.

(Barolo et al., 2002)

Binding of H peptide to GST-CtBP detected by [35]S-labeled streptavidin.

(Barolo et al., 2002)
FBrf0151327-4.PD.A
Reference
Barolo et al., 2002
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
CtBP 

dCtBP

bait

GST tag

H 

H

prey

[35]S label

H 
H 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
H 
CtBP interaction domain
sufficient binding region
SSSSASASSSAATSGASSSAMFHTSSLRNEQSSDLPLNLSKH
H 
CtBP interaction domain
necessary binding region
PLNLSKH
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Barolo et al., 2002)
FBrf0191199-2.coIP.WB
Reference
Nagel et al., 2005
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
H 

Hairless

bait
CtBP 

CtBP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Nagel et al., 2005)
FBrf0191199-6.Y2H
Reference
Nagel et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
H 

H

bait

fused to LexA DNA-binding domain

H 
H 
CtBP 

CtBP

prey

fused to VP16 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
H 
CtBP binding domain
necessary binding region
aa 1063-1077

coordinates relative to H-PA

H 
CtBP binding domain
mutation disrupting interaction
PLNLSKH

mutated to VIQITKR, "H*" construct, coordinates relative to H-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Nagel et al., 2005)

Two-hybrid system: yeast LexA-BD/VP16-AD

(Nagel et al., 2005)
FBrf0226611-3.Y2H
Reference
Nagel and Preiss, 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
H 

H

bait

fused to lexA DNA-binding domain

CtBP 

CtBP

prey

fused to B42 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Nagel and Preiss, 2014)
FBrf0237227-3.Y2H
Reference
Zehender et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
H 

H

bait

fused to lexA DNA-binding domain

CtBP 

CtBP

prey

fused to VP16 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Zehender et al., 2017)

Two-hybrid system: yeast lexA-BD/VP16-AD

(Zehender et al., 2017)
FBrf0246852-3.Y2H
Reference
Nagel et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
H 

H

bait

fused to lexA DNA-binding domain

H 
CtBP 

CtBP

prey

fused to VP16 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
H 
interaction domain residues 1071-1077
mutation disrupting interaction
aa 1071-1077

PLNLSKH to VIQITKR, coordinates relative to H-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Nagel et al., 2020)

Two-hybrid system: yeast lexA-BD/VP16-AD

(Nagel et al., 2020)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (6)