FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Su(dx), N
FlyBase ID
FBig0000103543
Interaction Network
Interactions Browser links
Su(dx) network
N network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0183818-1.PD.WB
Reference
Wilkin et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Su(dx) 

Su(dx)

bait

GST tag

Su(dx) 
N 

N

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Su(dx) 
WW1 and WW2 domains
sufficient binding region
aa 474-554
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected S2 cell extract.

(Wilkin et al., 2004)
FBrf0216227-1.PD.A
Reference
Jennings et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Su(dx) 

Su(dx)

bait

GST tag

Su(dx) 
Su(dx) 
Su(dx) 
Su(dx) 
N 

N

prey

[35]S label

N 
N 
N 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
N 
intracellular PY motif
sufficient binding region
aa 1763-2333
N 
intracellular PY motif
necessary binding region
aa 2325-2333
N 
intracellular PY motif Y2328F mutation
mutation decreasing interaction
aa 2328

Y2328F

Su(dx) 
WW-1 motif
sufficient binding region
aa 352-398
Su(dx) 
WW-2 motif
sufficient binding region
aa 392-431
Su(dx) 
WW-3 motif
sufficient binding region
aa 471-514
Su(dx) 
WW-4 motif
sufficient binding region
aa 515-554
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Jennings et al., 2007)
FBrf0216227-2.ITC
Reference
Jennings et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Su(dx) 

Su(dx)

bait
Su(dx) 
Su(dx) 
Su(dx) 
Su(dx) 
Su(dx) 
Su(dx) 
Su(dx) 
N 

N

prey
N 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
N 
PY domain
sufficient binding region
aa 2322-2333
Su(dx) 
WW-1 motif
sufficient binding region
aa 352-398
Su(dx) 
WW-2 motif
sufficient binding region
aa 392-431
Su(dx) 
WW-3 motif
sufficient binding region
aa 471-514
Su(dx) 
WW-4 motif
sufficient binding region
aa 515-554
Su(dx) 
WW-3 motif
mutation disrupting interaction
aa 505

W505F

Su(dx) 
WW-4 motif F549W
mutation increasing interaction
aa 549

F549W

Su(dx) 
WW-4 motif F549A
mutation disrupting interaction
aa 549

F549A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey peptide produced commercially.

(Jennings et al., 2007)
FBrf0216227-3.NMR
Reference
Jennings et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Su(dx) 

Su(dx)

neutral component
Su(dx) 

Su(dx)

neutral component
Su(dx) 
Su(dx) 

Su(dx)

neutral component
Su(dx) 
N 

N

neutral component
N 
N 

N

neutral component
N 

N

neutral component
N 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Su(dx) 
WW-4 motif
sufficient binding region
aa 523-552
N 
intracellular PY motif
sufficient binding region
aa 2323-2333
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Jennings et al., 2007)
FBrf0240436-2.PD.MW
Reference
Yao et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
N 

Notch

bait

GST tag

N 
Su(dx) 

Su(dx)

prey

MBP tag

Su(dx) 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
N 
PY domain
sufficient binding region
aa 2319-2333
Su(dx) 
LWW34 domains
sufficient binding region
aa 418-566
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Yao et al., 2018)
FBrf0240436-5.PD.MW
Reference
Yao et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Su(dx) 

Su(dx)

bait

MBP tag

Su(dx) 
N 

Notch

prey

GST tag

N 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
N 
PY domain
sufficient binding region
aa 2319-2333
Su(dx) 
LWW34 domains
sufficient binding region
aa 418-566
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Yao et al., 2018)

Addition of Ndfip competes with N for Su(dx) binding.

(Yao et al., 2018)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)