FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
N, mam
FlyBase ID
FBig0000103600
Interaction Network
Interactions Browser links
N network
mam network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0130233-3.Y2H
Reference
Petcherski and Kimble, 2000
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mam 

dMam

prey

fused to GAL4 activation domain

mam 
mam 
N 

N

ancillary

bridging factor

N 
N 

N

ancillary

bridging factor

N 

N

ancillary

bridging factor

N 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mam 
N-terminal conserved region
sufficient binding region
aa 1-198

coordinates relative to mam-PA

N 
intracellular domain
sufficient binding region
aa 1763-2224
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; ancillary factor produced as transgenic protein; prey produced as transgenic fusion protein (prey was produced as transgenic fusion protein.

(Petcherski and Kimble, 2000)

Three-hybrid system: yeast GAL4-BD/GAL4-AD and an additional ancillary bridging factor.

(Petcherski and Kimble, 2000)
FBrf0183909-1.coIP.MS
Reference
Veraksa et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
N 

NICD

bait

TAP tag

N 
mam 

Mam

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
N 
intracellular domain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of Kc cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Veraksa et al., 2005)

Positive control.

(Veraksa et al., 2005)
FBrf0183909-3.coIP.MS
Reference
Veraksa et al., 2005
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
N 

NICD

bait

TAP tag

N 
mam 

Mam

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
N 
intracellular domain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Veraksa et al., 2005)

Positive control.

(Veraksa et al., 2005)
FBrf0183909-5.coIP.MS
Reference
Veraksa et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mam 

Mam

bait

TAP tag

N 

Notch

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of Kc cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Veraksa et al., 2005)

Positive control.

(Veraksa et al., 2005)
FBrf0215044-6.Y2H
Reference
Maier et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mam 

mamN

bait

fused to LexA DNA-binding domain

mam 
N 

N-ANK

ancillary

bridging factor

N 

N-ANK

ancillary

bridging factor

N 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mam 
N-terminal region
sufficient binding region
aa 113-178
N 
ankyrin repeats
sufficient binding region
aa 1873-2176
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; ancillary factor produced as transgenic protein; prey produced as transgenic fusion protein (prey was produced as transgenic fusion protein.

(Maier et al., 2011)

Three-hybrid system: yeast GAL4-BD/GAL4-AD and an additional ancillary bridging factor.

(Maier et al., 2011)

Two-hybrid system: yeast LexA-BD/B42-AD

(Maier et al., 2011)
FBrf0232880-7.Y3H
Reference
Yuan et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
N 

N

bait

fused to LexA DNA-binding domain

N 
mam 

Mam

ancillary
mam 

Mam

ancillary
mam 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mam 
region
sufficient binding region
aa 118-194

coordinates relative to mam-PA

N 
intracellular domain
sufficient binding region
aa 1762-2176
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Three-hybrid system: yeast LexA-BD/B42-AD

(Yuan et al., 2016)

mam was co-expressed as a bridging factor in this three hybrid experiment, with N as bait and Su(H) as prey.

(Yuan et al., 2016)

Positive control.

(Yuan et al., 2016)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Yuan et al., 2016)
FBrf0236767-4.Y3H
Reference
Nagel et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mam 

Mam

bait

fused to LexA DNA-binding domain

mam 
N 

N

ancillary
N 

N

ancillary
N 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mam 
interaction domain
sufficient binding region
aa 118-194
N 
intracellular domain
sufficient binding region
aa 1762-2176
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Nagel et al., 2017)

Three-hybrid system: yeast LexA-BD/B42-AD

(Nagel et al., 2017)

N intracellular domain aa 1762-2176 was co-expressed as a bridging factor in this three hybrid experiment, with mam as bait and Su(H) as prey.

(Nagel et al., 2017)
FBrf0243779-3.Y3H
Reference
Gahr et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mam 

Mam

bait

fused to lexA DNA-binding domain

mam 
N 

RICN

ancillary
N 
N 

RICN

ancillary
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mam 
interaction domain
sufficient binding region
aa 118-194
N 
intracellular and RAM domain
sufficient binding region
aa 1762-2176
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Three-hybrid system: yeast lexA-BD/B42-AD, N ICD was coexpressed as a bridging factor in a yeast three-hybrid assay with mam as bait and Su(H) as prey. Positive control.

(Gahr et al., 2019)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Gahr et al., 2019)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (6)