Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.
Purified N intracellular domain protein was kinase labeled with [32]P in vitro.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.
Positive control.
Source was adult heads of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Source was embryos of wild-type or transgenic fly line; bait produced from endogenous gene; prey produced from endogenous gene or transgenic construct.
Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.