FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
H, run
FlyBase ID
FBig0000103854
Interaction Network
Interactions Browser links
H network
run network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0213441-2.Y2H
Reference
Walrad et al., 2011
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
run 

Runt

bait

fused to LexA DNA-binding domain

run 
H 

Hairless

prey

fused to VP16 activation domain

H 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
H 
C-terminal region
sufficient binding region
aa 672-1077

coordinates relative to H-PA

run 
VWRPY motif
necessary binding region
aa 485-509
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Walrad et al., 2011)

Two-hybrid system: yeast LexA-BD/VP16-AD

(Walrad et al., 2011)

The run bait lacks the "Runt domain" (aa 110-225), which is known to inhibit the run-gro interaction.

(Walrad et al., 2011)
FBrf0213441-3.coIP.WB
Reference
Walrad et al., 2011
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
H 

Hairless

bait
run 

Runt

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Walrad et al., 2011)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)