FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
H, gro
FlyBase ID
FBig0000103946
Interaction Network
Interactions Browser links
H network
gro network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0151327-1.PD.A
Reference
Barolo et al., 2002
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
H 

H

bait

GST tag

H 
H 
gro 

Gro

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
H 
Gro interaction domain
sufficient binding region
aa 618-723

coordinates relative to H-PA

H 
YSIHSLLG motif
necessary binding region
YSIHSLLG

mutated to AAAHSAAG

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Barolo et al., 2002)

gro binds H (Hairless), h (hairy) but not Su(H).

(Barolo et al., 2002)
FBrf0191199-1.coIP.WB
Reference
Nagel et al., 2005
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
H 

Hairless

bait
gro 

Gro

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Nagel et al., 2005)
FBrf0191199-3.coIP.WB
Reference
Nagel et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
gro 

Gro

bait
H 

H

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Binding of H to gro is independent of the presence of CtBP.

(Nagel et al., 2005)

Interaction in vitro; bait produced by in vitro translation; prey produced by in vitro translation.

(Nagel et al., 2005)
FBrf0191199-5.Y2H
Reference
Nagel et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
H 

H

bait

fused to LexA DNA-binding domain

H 
H 
H 
gro 

Gro

prey

fused to VP16 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
H 
Groucho binding domain
sufficient binding region
aa 565-712

coordinates relative to H-PA

H 
Groucho binding domain
necessary binding region
aa 490-712

coordinates relative to H-PA

H 
Groucho binding domain-residue 663
mutation disrupting interaction
aa 663

Y663A, "GBD-2" construct, coordinates relative to H-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Nagel et al., 2005)

Two-hybrid system: yeast LexA-BD/VP16-AD

(Nagel et al., 2005)
FBrf0226611-2.Y2H
Reference
Nagel and Preiss, 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
H 

H

bait

fused to lexA DNA-binding domain

gro 

gro

prey

fused to B42 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Nagel and Preiss, 2014)
FBrf0237227-2.Y2H
Reference
Zehender et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
H 

H

bait

fused to lexA DNA-binding domain

gro 

Gro

prey

fused to VP16 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Zehender et al., 2017)

Two-hybrid system: yeast lexA-BD/VP16-AD

(Zehender et al., 2017)
FBrf0246852-2.Y2H
Reference
Nagel et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
H 

H

bait

fused to lexA DNA-binding domain

H 
gro 

Gro

prey

fused to VP16 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
H 
interaction domain residue 663
mutation disrupting interaction
aa 663

Y663A, coordinates relative to H-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast lexA-BD/VP16-AD

(Nagel et al., 2020)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Nagel et al., 2020)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (5)