Soti
GFP tag
Cullin-3
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
Cul3T
klhl10 was co-transfected with Cul3 and soti, as well as skap in increasing amounts.
Binding of Cul3 to soti is inhibited by cotransfection of increasing amounts of skap.