Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus-infected Sf9 insect cell system; prey produced as a recombinant fusion protein in baculovirus-infected Sf9 insect cell system.

Components of the "WRC" complex were co-expressed in Sf9 cells (HSPC300, Sra-1, Abi, SCAR, Hem), pulled down via GST tag, and further purified by size exclusion column.

Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus-infected Sf9 insect cell system; prey produced as a recombinant fusion protein in baculovirus-infected Sf9 insect cell system.

Components of the "WRC" complex were co-expressed in Sf9 cells (HSPC300, Sra-1, Abi, SCAR, Hem), pulled down via MBP tag, and further purified by size exclusion column.