FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
mir-8, Ser
FlyBase ID
FBig0000104482
Interaction Network
Interactions Browser links
mir-8 network
Ser network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0213063-1.miR-mRNA.LUC
Reference
Vallejo et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
mir-8 

mir-8

bait
mir-8 
Ser 

Ser

prey
Ser 
Ser 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Ser 
3-prime UTR
sufficient binding region
Ser 
mir-8 target site
necessary binding region
AGCAGUUACCGUUAUUGUAUUUUGUCUAUAGUUAGAUUGGUAGUAUUA
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

luciferase reporter assay

(Vallejo et al., 2011)

Source was S2 cell line; bait produced from transfected construct; prey produced from transfected reporter construct.

(Vallejo et al., 2011)
FBrf0213063-2.miR-mRNA.GFP
Reference
Vallejo et al., 2011
Description
physical association
Assay
Collection
Source/Stage
wing disc / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mir-8 

mir-8

bait
mir-8 
Ser 

Ser

prey
Ser 
Ser 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Ser 
3-prime UTR
sufficient binding region
Ser 
mir-8 target site
necessary binding region
AGCAGUUACCGUUAUUGUAUUUUGUCUAUAGUUAGAUUGGUAGUAUUA
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

GFP reporter assay

(Vallejo et al., 2011)

Source was larval wing discs of transgenic fly line; bait produced from transgenic construct; prey produced from transgenic reporter construct.

(Vallejo et al., 2011)
FBrf0213063-3.miR-mRNA.IHC
Reference
Vallejo et al., 2011
Description
physical association
Assay
Collection
Source/Stage
wing disc / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mir-8 

mir-8

bait
Ser 

Ser

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Mutation of mir-8 increases Ser protein levels (but does not affect Ser mRNA levels).

(Vallejo et al., 2011)

Source was larval wing discs of mutant fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Vallejo et al., 2011)
FBrf0213063-4.miR-mRNA.IHC
Reference
Vallejo et al., 2011
Description
physical association
Assay
Collection
Source/Stage
wing disc / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mir-8 

mir-8

bait
Ser 

Ser

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Overexpression of mir-8 reduces Ser protein levels.

(Vallejo et al., 2011)

Source was larval wing discs of transgenic fly line; bait produced from transgenic construct; prey produced from endogenous gene.

(Vallejo et al., 2011)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)