FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Rpt2, H
FlyBase ID
FBig0000104500
Interaction Network
Interactions Browser links
Rpt2 network
H network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0190959-1.Y2H
Reference
Müller et al., 2006
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
H 

Hairless

bait

fused to LexA DNA-binding domain

H 
Rpt2 

Pros26.4

prey

fused to B42 activation domain

Rpt2 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
H 
CX domain
necessary binding region
aa 712-1037

coordinates relative to H-PA

Rpt2 
N-terminal region
sufficient binding region
aa 1-193

"S4-I" construct, coordinates relative to Rpt2-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Müller et al., 2006)

Two-hybrid system: yeast LexA-BD/B42-AD

(Müller et al., 2006)
FBrf0190959-2.coIP.WB
Reference
Müller et al., 2006
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rpt2 

S4

bait
H 

H

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Müller et al., 2006)
FBrf0226611-4.Y2H
Reference
Nagel and Preiss, 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
H 

H

bait

fused to lexA DNA-binding domain

Rpt2 

Rpt2

prey

fused to B42 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Nagel and Preiss, 2014)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)